Older Whooping Cough Vaccine May Actually Deliver Longer Protection
Researchers at La Jolla Institute for Allergy and Immunology (LJI) reported today that individuals who had been inoculated with the newer pertussis vaccine mount a weaker recall response when receiving booster shots later on.
Traditionally the ability to induce a high antibody titer has been the yardstick against which vaccines have been measured.
"We see a dramatic imprinting effect depending on whether people received the old or the new pertussis vaccine as part of their original childhood immunizations," says the study's senior author LJI professor Alessandro Sette, Dr. Biol. Sci., head of the Division of Vaccine Discovery, in a press release.
"Even if the booster shots they received as middle and high schoolers or as adults contained the same vaccine the immune system still has a very striking recollection of what it saw during the first immunization."
Unraveling the differences between the two vaccines is key to an understanding of how to better prevent whooping cough and may also provide important lessons on vaccine efficacy in general, potentially applicable to other vaccines.
Specifically, this study found that the new vaccine fell short of generating a robust T cells response, which provides the long-term memory that allows the immune system to mount a rapid response if exposed to the pathogen.
"Ideally, you should engage both arms of a protective response against pathogens--B cells that produce antibodies and T cells that generate long-term memory," says first author Ricardo Antunes, Ph.D., a postdoctoral researcher in the Sette lab.
"But apparently the new vaccine fails to generate an adequate T cell response."
"Although B cells are a very important component of vaccine efficacy, the important role of T cells is being more and more appreciated and the key point of our study is to show that there are striking differences in the T cell response to the two different vaccines," says Antunes.
Bordetella pertussis, the bacteria that causes whooping cough, produces a toxin that causes uncontrollable, sometimes deadly coughing fits.
Before a vaccine became available, whooping cough killed thousands of people and caused hundreds of thousands to become ill year after year.
Despite the added booster shots, whooping cough cases in recent years began to increase, and by 2015 more than 20,000 cases were reported nationwide.
With the introduction of the first vaccine, which was crafted from dead bacteria, cases dramatically declined but unwanted side effects from the whole cell (wP) preparation led the U.S. and many other countries to switch to acellular (aP) formulas that relied on purified bacterial proteins to induce immunity.
Vaccination against the disease currently involves a series of five shots given to young children at 2, 4 and 6 months of age, somewhere between 15 and 18 months, and a fifth dose between 4 and 6 years old and a final booster between 11 and 12 years old.
Pregnant women receive a single shot in the third trimester of pregnancy to protect newborns, the population at highest risk for pertussis complications.
Since the birth years of the teenagers and young adults most affected by the sudden increase in pertussis cases coincided with the nationwide switch from the wP to the aP vaccine, the question arose whether the new vaccine formulation afforded less protection.
To look for answers, the LJI team recruited 114 healthy adults who had been originally vaccinated either with wP or aP in infancy and administered booster vaccinations with aP in middle and high school and as adults and analyzed their immune response at regular intervals.
Their results clearly showed that priming in the first few months after birth with the aP or wP vaccines induces different T cell responses.
Although both are initially capable of generating protective immunity, differences evolve over more than 15 years.
In addition to more subtle shifts in B cell populations and cytokine secretion, T cells originally primed with aP gradually lose the ability to respond to booster vaccination. "These cells just sit there and do nothing while T cells primed with wP respond with a pronounced boost," says Antunes. Detailed transcriptomics analyses revealed the diminished activity of genes associated with cell proliferation.
"Since we can see clear differences in the immune signatures induced by the two different vaccines within days of a booster, this suggests that a potential path forward would be to test if new vaccines might be able to reproduce the more protective signature," says Sette.
These researchers did not disclose conflicts of interest: Ricardo da Silva Antunes, Mariana Babor, Chelsea Carpenter, Natalie Khalil, Mario Cortese, Alexander J. Mentzer, Gregory Seumois, Christopher D. Petro, Lisa A. Purcell, Pandurangan Vijayanand, Shane Crotty, Bali Pulendran, Bjoern Peters and Alessandro Sette.
The study was funded in part by La Jolla Institute for Allergy and Immunology and the National Institute of Allergy and Infectious Diseases (HHSN272200900044C, NIH U19 AI118626, and NIH S10OD016262.)